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D, without magnesium chloride, Sigma-Aldrich , 1. In some embodiments, PCR may be performed using a single amplification primer that is complementary to the constant end sequence of the library molecules. In an effort to minimize the number of primers to investigate, and provide a workable example, it was decided to limit the number of primers to evaluate to Such primers would be able to hybridize to a maximal number of sequences throughout the target nucleic acid, while the tendency to self-hybridize or cross-hybridize with other primers would be minimized. Non-limiting examples of suitable universal priming sites include T7 promoter sequence, T3 promoter sequence, SP6 promoter sequence, M13 forward sequence, or M13 reverse sequence. Alternatively, the primed target nucleic acid may be replicated via a two-step process.

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